Fig. 2

Overexpression of miR-22 inhibited HCC cell proliferation and metastasis in vitro. a Transfection of miR-22 overexpression vector to MHCC-97H and SMMC-7721 cells increases the expression of miR-22 detected by real-time quantitative RT-PCR. b Cell proliferation of these cells transfected as in a was measured at the indicated time periods using the MTT assay. c Overexpression of miR-22 presented a slower closing of scratch wound, compared with pcDNA3.1 control, at 48 h after transfection in MHCC-97H and SMMC-7721 cells. d Migration and invasion potential in both cell lines treated as above were measured by Transwell assays and were expressed as relative cell numbers. Scale bars, 50 μm. Scale bars, 50 μm. *P < 0.05