Fig. 5

Co-treatment of TNF-α and 5-FU induced necroptosis in MDA-MB-231 breast cancer cells. a Cell cycle distribution after treatment with TNF-α (5 ng/ml) and/or 5-FU (12 μM). b Apoptosis/necrosis rate after treatment with TNF-α and/or 5-FU. c Protein level of necroptosis marker, RIP3, after treatment with TNF-α and/or 5-FU. d Confirmation of knockdown of RIP3 by using real-time PCR. e Effect of RIP3 knockdown on the synergetic effect of TNF-α and 5-FU. **P < 0.01 indicated significant differences from the respective control groups; ^## P < 0.01 indicated significant differences from the TNF-α (5 ng/ml) treated groups; ^& P < 0.05, ^&& P < 0.01 and ^@@ P < 0.01 indicated significant differences between folds induction