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Fig. 1 | Cancer Cell International

Fig. 1

From: Up-regulation of CRKL by microRNA-335 methylation is associated with poor prognosis in gastric cancer

Fig. 1

Expression of miR-335 and CRKL in GC cell lines and tissues. a Analysis of miR-335 expression levels in cell lines by qRT-PCR. MiR-335 expression levels in four GC cell lines were significantly down-regulated compared with levels in normal gastric mucosal epithelial cells (GES-1) (P < 0.01). Each bar represents fold change relative to GES-1 cells calculated by \(2^{{ - \Delta \Delta {\text{CT}}}}\). **P < 0.01 between groups. b Expression levels of MiR-335 in paired GC and adjacent normal tissues. Quantification of miR-335 by qRT-PCR. c Analysis of transcription levels of CRKL in cell lines by qRT-PCR. There was no significant difference in CRKL mRNA levels between the four GC cell lines (SGC-7901, MKN-45, BGC-823, and AGS) and GES-1 cells (P > 0.05). d CRKL protein expression levels in GC cell lines (SGC-7901, MKN-45, BGC-823, and AGS) was significantly up-regulated compared with GES-1 cells, determined by western blot. Numbers above the blot indicate normalized protein amounts relative to the negative control, as determined by densitometry

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