Fig. 2

Rab27B is a target of miR-20a-5p in NPC cells. Level of miR-20a-5p,Rab27B mRNA and protein levels in the miR-20a-5p mimic (5PM)-transfected CNE-2 and the miR-20a-5p antagomiR (5PA)-transfected CNE-1 cells versus the negative control (NC) cells, as determined by qRT-PCR (a, b) and western blot analyses (c). The perfectly matched region of Rab27B gene 3'-UTR with miR-20a-5p (d). The relative luciferase activity (fold) of the reporter with wild-type (WT) Rab27B-UTR were determined in the miR-20a-5p mimic or anti or Controls transfected NPC cells. The reporter without Rab27B-UTR (Vec) was used as a reference. The Renilla luciferase activity of a co-transfected control plasmid was used to control the transfection efficacy (e). The representative results from three independent experiments shown. *P value < 0.05; **P value < 0.01