Fig. 3
From: MiR–20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells
Effects of a forced reversal of the miR-20a-5p or Rab27B levels on the radio-resistance of CNE-1 and CNE-2 cells. MiR-20a-5p mimic (5PM)-transfected CNE-2 increases NC cells resistance to radiation treatment versus the negative control (NC) cells (a). Rab27B protein level (western blot analysis) and mRNA determined by qRT-PCR in the si-Rab27B-transfected versus the NC-transfected CNE-2 cells treated with a 4-Gy dose of radiation (b, c). Si-Rab27B-transfected CNE-2 increases NC cells resistance to radiation treatment versus the negative control (NC) cells (d). MiR-20a-5p antagomiR (5PA)-transfected CNE-1 decreases NC cells resistance to radiation treatment versus the negative control (NC) cells (e). Expression of Rab27B in overexpression construct transfected CNE-1 cells. Representative areas of CNE-1 cells transfected with GFP-Rab27B ectopic expression construct were shown and GFP was used as a negative control (f). Rab27B mRNA level (qRT-PCR analysis) in the GFP-Rab27B-transfected versus the GFP-transfected CNE-1 cells treated with a 4-Gy dose of radiation (g). GFP- Rab27B-transfected CNE-1 decreases NC cells resistance to radiation treatment versus the negative control (GFP) cells (h)