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Fig. 1 | Cancer Cell International

Fig. 1

From: Global expression profile of tumor stem-like cells isolated from MMQ rat prolactinoma cell

Fig. 1

Overview of gene expression array. a Normal distribution of RNAs in classical MMQ cells and MMQ TSLCs. The Log2 absciss are presented the differences existing between the 2 group. The vertical axis showed the number of probe. In general, the figure become approximately normally distribution, which showed the up-regulated RNA should be roughly the same as the down-regulated RNA. b Volcano Plots of RNAs in classical MMQ cells and MMQ TSLCs. The Log2 absciss are presented the differences existing between the 2 group. The vertical axis showed the P value, which represented the significance of the difference. The red line mean the P = 0.05. The inclusion criteria of alternative gene: the expression of the gene was changed more than 2.0 folds (the green line) and the P value was less than 0.05 (the red line). Microarray showed that 566 known RNA were over-expressed and 532 known RNA were low-expressed in the MMQ TSLCs. c Biological processes analysis of the gene expression profiles. 15 pathway were shown to be significantly regulated with more than 500 genes differential expression in two group. d The cluster analysis of classical MMQ cells and MMQ TSLCs. Further study in pathway in cancer and cell cycle between MMQ cells and TSLCs were taken into consideration. According to the clustering analysis of three times independent microarray experiments, 82 candidate genes were selected. E. To verify the 82 candidate genes, MMQ cells and MMQ TSLCs were used to perform qRT-PCR for the identified RNAs. 15 differential expressed genes existed steady difference. *P < 0.05, **P < 0.01. TSLC tumor stem-like cell

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