Fig. 5
From: PI3Kα isoform-dependent activation of RhoA regulates Wnt5a-induced osteosarcoma cell migration
RhoA acts as the downstream of Wnt5a/PI3K/Akt signaling. a Human osteosarcoma cells MG-63 were transfected with RhoA-siRNA or scrambled siRNA (Ctrl) for 48 h, then were treated with 100 ng/mL of Wnt5a and harvested at 30 min after the start of Wnt5a treatment for SDS-PAGE and immunoblot analysis with antibodies to p-Akt and total Akt. p-Akt/total Akt ratios were normalized to the average value of Ctrl group. Data were presented as mean ± SD of 3 determinations. b, c MG-63 (b) and U20S cells (c) were transfected with DN-RhoA (GFP-RhoA-N19) or vector (Ctrl) for 48 h, then were treated with 100 ng/mL of Wnt5a and harvested at 30 min after the start of Wnt5a treatment for SDS-PAGE and immunoblot analysis. p-Akt/total Akt ratios were normalized to the average value of Ctrl group. Data were presented as mean ± SD of 3 determinations. d A schematic illustration of the regulation of RhoA in Wnt5a/PI3K/Akt signaling (See text for detail)