Human U87 glioblastoma cells with stemness features display enhanced sensitivity to natural killer cell cytotoxicity through altered expression of NKG2D ligand

Table 1 NK ligand primers used for RT-PCR

NK receptor	Ligands	Forward primer	Reverse primer	Amplicon size (bp)
NKG2D	MICA	TCTACTACGATGGGGAGCTCTT	ACTGGGGCATTGTCCATTC	66
	MICB	CTGAGAAGGTGGCGACGTA	CGAAGACTGTGGGGCTCA	111
	ULBP-1	TGGGGGATTGTAAGATGTGG	GGCCAGAGAGGGTGGTTT	83
	ULBP-2	CCGCTACCAAGATCCTTCTG	TGACGGTGATGTCATAGCAAA	105
	ULBP-3	AGGAAGAAGAGGCTGGAACC	CTATGGCTTTGGGTTGAGCTA	70
	ULBP-4	GGGAGAATTGACCCAAACG	CTTGCAGAGTGGAAGGATCAC	104
NKG2A/C/E/H	HLA-E	GGAGTGGCTCCACAAATACC	GTCAGAGATGGGGTGGTGAG	94
2DL4	HLA-G	CTGGAGAACGGGAAGGAGAT	GGGTGGCCTCATAGTCAAAG	88
2B4	CD48	CCTACATCATGAGGGTGTTGAA	ATGACAGGCTTGGGTACAGG	91
NTBA	NTBA	CCACAATTAATCATTCCAAAGAGA	TGTCATTCCCGAATTCCTCT	107
LIR2	HLA-F	ATCACCCAGCGCTTCTATGA	CTGGGGAGACTGCTCTGC	138
NKR-P1A	LLT-1	GTTCAGGGCCAGTGCATATT	GACTCTGCACCTTCCTTCACA	61
DNAM-1	PVR-1	CAACGTCACCAATGCCCTA	CTGAGTGCTCACTGGGAGGT	80
	PVR-2	GATATCTGGCTCCGAGTGCT	TCCAGTGAGCTGGACCTTCT	69
	Nectin2-1	GGCAGAGGAGGACGAAGAC	GAGCTGGGAGGGCATCTC	94
	Nectin2-2	GAGGACGAGGGCAACTACAC	GGCCTCAGCTTGGTTCTTG	108
CD100	CD72	TTCTTCACATGCGGCTCA	AGCAGCTTTTCTGATGCATTATC	67
CD28	CD80	TCCTGGGCCATTACCTTAATC	CATCTTGGGGCAAAGCAG	77
LFA-1	ICAM-1	CCTTCCTCACCGTGTACTGG	AGCGTAGGGTAAGGTTCTTGC	90
LFA-2	LFA-3	CATGTATTGTGCTGTATATGAATGGT	TCTGGCTTCCCAAGTAATGG	141
CRACC	CRACC	TCTACTATGTGGGGATATACAGCTCA	TTTAGGCTTTGACAGGTGCTC	92
FasL	Fas	GTGGACCCGCTCAGTACG	TCTAGCAACAGACGTAAGAACGA	112
TRAIL	DR4	GGGTCCACAAGACCTTCAAGT	GGGTCCACAAGACCTTCAAGT	68
	DR5-1	GTGTGTCAGTGCGAAGAAGG	GACCATCCCTCTGGGACA	90
	DR5-2	GCACCAGGTGTGATTCAGG	CCCTCTGGGACACCCTGT	143
TNF	TNFR1	CTCTCCACCGTGCCTGAC	CCAGTCCAATAACCCCTGAG	85

CRACC CD2-like receptor activating cytotoxic cell, DNAM-1 DNAX accessory molecule-1, ICAM-1 intercellular adhesion molecule 1, LFA lymphocyte function-associated antigen, MIC MHC I-related chain, NK natural killer, NKG2D NK receptor group 2; membrane D, PVR-1 poliovirus receptor-1, RT-PCR real-time quantitative polymerase chain reaction, TNF tumor necrosis factor, TRAIL tumor necrosis factor-related apoptosis-inducing ligand, ULBP UL16 binding protein

ISSN: 1475-2867