Fig. 1

rs35301225 in miR-34a on the regulatory on E2F1 expression and cell proliferation. a Cell proliferation was measured by CCK8 assay in Hct-116 and sw-480 cell lines. Cells were treated with miR-34a harboring CC or AA genotype and control by vector transfection. b S phase of Hct-116 and sw-480 treated with miR-34a harboring CC or AA genotype was determined by flow cytometry. c Expression of E2F1 in of Hct-116 and sw-480 treated with miR-34a harboring CC or AA genotype were determined by western-blot. d Cells were co-transfected with miR-34a with CC or AA genotype, Renilla luciferase vector pGL3-Luc for 48 h. Both firefly and Renilla luciferase activities were measured in the same sample. Firefly luciferase signals were normalized with Renilla luciferase signals. Data was presented as the mean ± SEM. *P < 0.05 and **P < 0.01