Fig. 4

ERK blockade rescues BPIQ-induced apoptosis of NSCLC cells. Cells were pre-incubated for 2 h with the following specific MAPK ERK inhibitors, PD98059 prior to BPIQ administration (see “Methods” section). Subsequently, the apoptotic populations induced by BPIQ were determined using flow cytometer-based Annexin V/PI staining. a Results of Annexin V/PI staining and b the quantitative analysis. Data are presented as mean ± SD. of at least three experiments independently. The results were analyzed with the statistical approach Student’s t-test (*p < 0.05 BPIQ vs. BPIQ with inhibitors pre-treated)