Fig. 4
From: HLBT-100: a highly potent anti-cancer flavanone from Tillandsia recurvata (L.) L.
Effect of HLBT-100 on cell viability. Cells were seeded at 2000–5000 cells/well in 96-well plates. After an overnight incubation, cells were treated with HLBT-100 a IMR-32 nueroblastoma cancer cell line and b neuroblastoma normal cells, and incubated for 72 h at 37 °C. The cell viability was determined using the Cell Titer-Glo luminescent cell viability assay kit after staining for 10 min, according to the manufacturer’s instructions. The IC50 values were generated using graphpad prism. Staurosporine (graphs on the right) was used as a positive control against both cell lines. Asterisk Lower concentrations of HLBT-100 versus control are slightly above 100% of control because of “edge effect” where slight evaporation of media in control wells on the edge of plate might result in lower OD values for those wells