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Fig. 8 | Cancer Cell International

Fig. 8

From: HLBT-100: a highly potent anti-cancer flavanone from Tillandsia recurvata (L.) L.

Fig. 8

The Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega) was used to detect caspase-3/7 activity of HLBT-100 based on the cleavage of a pro-fluorescent DEVD peptide-rhodamine 110 substrate [(Z-DEVD)2-R110] according to manufacturer’s instruction and as reported by Wagner et al. [78]. PC-3 prostate cancer cell line was cultured in F12 K medium supplemented with 10% FBS and 100 µg/ml penicillin, and 100 µg/ml streptomycin. PC-3 cells were treated with HLBT-100 or reference compound staurosporine for 6 h with serum free medium. The activation was considered significant only when the highest compound dose induced caspase-3/7 activity ≥200% compared to DMSO

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