Fig. 1

Inhibitory effect of LEE011 on leukemia cell growth. a Western blot analysis showing the expression of CDK6, CDK4, Cyclin D1 and Cyclin D2 in nine leukemia cell lines. Our results indicated that there is positive correlation between IC50 and the expression of Cyclin D1. b Molecular structure of LEE011. c Proliferation and IC50 analysis of LEE011 in six leukemia cells. IC50s: U937 3.7 µM, HL60 0.5 µM, NB4 2.6 µM, MV4-11 2.1 µM, K562 13.1 µM and CCRF 8.6 µM. d Micrographs of HL-60 and MV4-11 cells treated with LEE011 (2 and 5 µM) or DMSO. e The IC50 of LEE011 in primary ALL and AML cells was also analyzed. In primary ALL cells, the IC50 of LEE011 was 1.73–14.68 µM; in primary AML cells the IC50 of LEE011 was 1.94–8.46 µM. All experiments were performed in quadruplicate. *P < 0.05, **P < 0.01