Fig. 4
Cell cycle analysis of leukemia cells treated with LEE011. Cell cycle analysis showing cells treated with LEE011 at 2 or 5 µM for 48 h, in eight leukemia cell lines. LEE011 significantly induced cell cycle G1 arrest. The G1 phase cell percentage following 5-µM LEE011 treatment of K562 cells was 81.23 ± 3.84% vs. DMSO group 32.46 ± 2.21%, P < 0.01. The G1 phase cell percentage in CCRF cells treated with 5 µM LEE011 was 89.83 ± 2.67% vs. DMSO group 45.80 ± 3.24%, P < 0.01. The G1 phase cell percentage in NB4 cells treated with 5 µM LEE011 was 94.79 ± 4.93% vs. DMSO group 45.59 ± 3.12%, P < 0.01. The G1 phase cell percentage in HL-60 cells treated with 5 µM LEE011 was 95.50 ± 2.97% vs. DMSO group 47.40 ± 3.00%, P < 0.01. The G1 phase cell percentage in MV4-11 cells treated with 5 µM LEE011 was 83.82 ± 2.81% vs. DMSO group 44.66 ± 1.90%, P < 0.01. The G1 phase cell percentage in SHI-1 cells treated with 5 µM LEE011 was 87.39 ± 2.80% vs. DMSO group 45.25 ± 4.61%, P < 0.01. The G1 phase cell percentage in THP-1 cells treated with 5 µM LEE011 was 50.51 ± 8.17% vs. DMSO group 45.64 ± 6.46%, P = 0.466. The G1 phase cell percentage in U937 cells treated with 5 µM LEE011 was 89.99 ± 3.54% vs. DMSO group 54.26 ± 2.92%, P < 0.01. **P < 0.01