Fig. 8

Molecular mechanism analysis of cellular senescence induced by LEE011 in leukemia cells. a Dysregulated genes, KIF20A and MYBL2, identified from gene arrays were confirmed by western blot analysis. b RNA interference of MYBL2 significantly down regulates the expression of MYBL2 in HL-60 cells. Proliferation analysis of Si-MYBL2 at 4 days showed an OD450 value of 0.79 ± 0.29 vs. Si-Nc 2.43 ± 0.43, P < 0.01. Apoptosis analysis showed Si-MYBL2 was 9.06 ± 1.62% vs. Si-Nc 4.36 ± 0.31%, P = 0.034. c RNA interference significantly downregulates the expression of PLK1 in HL-60 cells. Proliferation analysis of Si-MYBL2 at 4 days showed an OD450 value of 0.67 ± 0.29 vs. Si-Nc 2.43 ± 0.43, P < 0.01. Apoptosis analysis showed Si-MYBL2 was 25.26 ± 4.91% vs. Si-Nc 3.67 ± 0.89%, P < 0.01. d RNA interference significantly downregulates the expression of KIF20A in HL-60 cells. Proliferation analysis of Si-MYBL2 at 4 days showed an OD450 value of 1.13 ± 0.39 vs. Si-Nc 2.43 ± 0.43, P < 0.05. Apoptosis analysis showed Si-MYBL2 was 11.9 ± 2.35% vs. Si-Nc 4.13 ± 0.81%, P = 0.02. *P < 0.05; **P < 0.01