Fig. 3

EphB2 knockdown combined with ionizing radiation results in G2/M arrest in medulloblastoma cells. a Increased accumulation of DAOY cells in G2/M phase of the cell cycle is evident in flow cytometry analyses following combined EphB2 knockdown and ionizing radiation. The cells were analyzed at 72 h post-radiation. The experiment was repeated at least two to three times. Representative plot is shown for DAOY cell line. b An increase in the expression of CyclinB1 is observed in DAOY cells transfected with EphB2-specific siRNA and exposed to 8 Gy dose of radiation. c Densitometric analysis was performed to quantify Cyclin B1 expression across different treatment and control groups