Fig. 5

EphB2 knockdown in combination with ionizing radiation results in decreased cell invasion in medulloblastoma cells. EphB2 knockdown when combined with radiation significantly decreases cell invasion compared to non-irradiated groups in DAOY (a) and UW228 (b) cells. Cell invasion was measured in a transwell chamber using electrical impedance method. Cell index values represent changes in electrical impedance across the membrane separating the upper and lower chambers, and show a direct correlation with the number of cells that have invaded into the lower chamber. Background invasion was subtracted to obtain normalized cell index values. Representative plot is shown in this figure for each individual cell line. Each experiment was repeated at least two times. **p < 0.005