Fig. 5

Association between NF-κB modulation and proteasome activity. a H125 cells pretreated with a non-cytotoxic dose of Bortezomib were exposed to CIGB-300. Nuclear extracts were prepared, resolved in 10% SDS-PAGE and blotted with anti p65/RelA antibodies (50 μg protein/lane). ACTIN expression level was used as protein loading control. Results are representative of three independent experiments. b Proteasome activity determination following the Proteasome-Glo™ cell-based assay for H125 treated with CIGB-300 or Bortezomib (Bz) as positive control. Data was expressed as the mean ± SE of three independent experiments. *p < 0.05 vs. Bz treated cells and ***p < 0.001 vs. control treated cells (one-way ANOVA test). c Cellular distribution of CIGB-300 (green, FITC) and α7/C8 protein (red, Alexa647) was visualized by confocal microscopy (top). Figure shows representative images of three independent experiments. Single cell analysis of Pearson and Manders coefficients were performed, as well as a representative colocalization map and a dot plot for both probes (bottom). Coefficient values are expressed as the mean ± SE of three independent experiments