Fig. 1

Expression of miR-338-3p and SphK2 in NSCLC. Relative expression of miR-338-3p was measured by qRT-PCR assay. U6 snRNA served as an endogenous control for normalization. SphK2 protein expression was measured using Western blot; GAPDH was an endogenous reference. Ratio of SphK2/GAPDH was equal to the protein expression level of SphK2 divided by the GAPDH protein expression level. a Western blot for SphK2 expression in four pairs of NSCLC tissues (T) and corresponding distant normal tissues (N). b Ratio of SphK2/GAPDH differed significantly between cancer tissues and corresponding distant normal tissues (*p < 0.05). c Ratio of SphK2/GAPDH in I + II stage cancer tissues and III stage cancer tissues of NSCLC with different TNM stages (*p < 0.05). d miR-338-3p expression differed significantly between cancer tissues and corresponding distant normal tissues (*p < 0.05). e miR-338-3p expression in I + II stage cancer tissues and III stage cancer tissues of NSCLC with different TNM stages (*p < 0.05). f Expression of SphK2 and miR-338-3p in NSCLC was negatively correlated. g Protein expression of SphK2 differed significantly between NHBE cell line and human lung cancer cell lines. h Relative expression of miR-338-3p differed significantly between NHBE cell line and human lung cancer cell lines