Fig. 3

Metformin ameliorated the process that insufficient RFA enhanced the growth of HCC cells in vivo. HepG2 and HepG2-H cells were injected subcutaneously into the upper right flank region of nude mice, treated with or without metformin, and tumor volume was measured. a Tumor volume was measured with a caliper rule every 3 days. Data were presented as the mean tumor volumes of mice. b Tumor size of the 24th day after cell implantation was displayed. c Tumor sections were stained for Ki-67, E-cadherin, and CD31. Representative images of the immunohistochemistry assay were shown (200×)