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Fig. 1 | Cancer Cell International

Fig. 1

From: Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation

Fig. 1

Knockdown of PLOD2 inhibits cell migration and invasion. a Knockdown of PLOD2 in HeLa and SiHa cells. Cells were transfected with si-scramble or siPLOD2 and then subjected to a Western blot analysis and qRT-PCR; α-tubulin and GAPDH were used as loading controls, respectively. b, d Depletion of PLOD2 inhibited cell migration and invasion. Si-scramble and siPLOD2 cells were subjected to transwell migration (b) and matrigel-coated invasion assays (d). Photos were captured under an ×200 objective lens. c, e Statistical analysis of migration (c) and invasion (e) based on the mean ± SD of at least three independent experiments. p values were obtained using Student’s t test. p < 0.05 is indicated by “**”, and p < 0.001 is indicated by “***”, si-scramble versus siPLOD2-s2 or siPLOD2-s4. f Scratch assays corroborated the decrease in HeLa and SiHa cell migration after the knockdown of PLOD2. Images were captured at ×200 magnification. g Statistical data were obtained as described above

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