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Fig. 6 | Cancer Cell International

Fig. 6

From: Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation

Fig. 6

PLOD2 is required for the adhesion interactions necessary for migration and invasion and acts by promoting the formation of focal adhesions. a The adhesion of HeLa and SiHa cells to matrix assessed 2 h after plating. Representative phase-contrast images of control and siPLOD2 cells are shown. b Statistical analysis of adhesion (a) based on the mean ± SD of at least three independent experiments. p values were obtained using Student’s t test. p < 0.05 is indicated by “**”, and p < 0.001 is indicated by “***”. c, d Western blots showing the expression of total FAK and phospho-FAK in control cells and siPLOD2 cells (c) or cells treated with minoxidil (0.5 mM) (d). e P-FAK immunofluorescent staining and phalloidin staining of HeLa and Siha cells. Arrows indicate focal adhesions, as confirmed by co-staining for p-FAK. Note the intense staining at the leading edge of invasive pseudopod protrusions, indicated by arrows. Photos were captured under an oil lens (×1000)

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