Fig. 4

27-OHC induces cell proliferation via ERβ. Cell proliferation assay in RWPE-1 (a), LNCaP (b) and PC3 (c) cells demonstrates attenuation of 27-OHC-induced cell proliferation with PHTPP treatment. Cells were treated with 1 µM 27-OHC and 10 µM PHTPP. Readings were recorded 48 h after treatments. Data is expressed as mean ± SEM. **p < 0.01; ***p < 0.001 versus controls, ^##p < 0.01; ^###p < 0.001 versus 27-OHC only treatment