Table 3 Primers used in determining mRNA expression of significantly regulated genes
Gene | Sequence |
|---|---|
CBX1-f | TGAGCAGCGTCACCCTTTACAC |
CBX1-r | CCACTTTGCCCTTTACCACTCG |
CBX5-f | TGGCACAATCTTGGCTTACTGT |
CBX5-r | ATGGTGGCACACACCTGTAGTC |
CTGF-f | AGGATGTGCATTCTCCAGCCATC |
CTGF-r | TGTCAGAGCTGAGTCTGCTGTTC |
CYP27A1-f | CAGCTGCGCTTCTTCTTTCAGC |
CYP27A1-r | TGGCCTTGTAAAGCACCTGTAAC |
FOXA1-f | TCCTCAGGAATTGCCCTCAAGAAC |
FOXA1-r | ATGACATGACCATGGCACTCTGC |
GLI2-f | TGTCTGAGTGACACCAACCAGAAC |
GLI2-r | TGTGAATGGCGACAGGGTTGAC |
GLI3-f | CCTCCAGCACCACTTCTAATGAGG |
GLI3-r | TCTGTGGCTGCATAGTGATTGCG |
IGFBP3-f | TCCAAGCGGGAGACAGAATATGG |
IGFBP3-r | AGGAACTTCAGGTGATTCAGTGTG |
INSIG2-f | CATGCCAGTGCTAAAGTGGATTTC |
INSIG2-r | TGGATAGTGCAGCCAGTGTGAG |
MDM2-f | TCCTCTCAAGCTCCGTGTTTG |
MDM2-r | TCATGATGTGGTCAGGGTAGATG |
MTA3-f | GCAGCAGAAGCTGAGAGTAAACTG |
MTA3-r | TGGTTGGGATTTGGTTTGCTGTAG |
NR1H2-f | GCATCCACTATCGAGATCATGCTG |
NR1H2-r | GAAGGTGATACACTCTGTCTCGTG |
OSBP-f | CTATGAAAGCCACAGAGGATGGC |
OSBP-r | GTCCTTCTTCCGCTCAAACCAC |
PTCH1-f | TCACCGTTCACGTTGCTTTGGC |
PTCH1-r | AAACATGTGCTCCAGGGCAAGC |
RXRB-f | TGCTGTGGAACAGAAGAGTGACC |
RXRB-r | ATGTTAGTCACAGGGTCATTTGGG |
SIRT1-f | ACAGGTTGCGGGAATCCAAAGG |
SIRT1-r | CCTAGGACATCGAGGAACTACCTG |
SPARC-f | TGGCGAGTTTGAGAAGGTGTGC |
SPARC-r | TGGCAAAGAAGTGGCAGGAAGAG |
SRD5A2-f | ACATACGGTTTAGCTTGGGTGTC |
SRD5A2-r | TTTCTCCAGGCTTCCTGAGCTG |
SREBF1-f | GCCATGGATTGCACTTTCGAAGAC |
SREBF1-r | GGTCAAATAGGCCAGGGAAGTCAC |
TAF3-f | GCCATCGGTACTCTGAGCTCTATG |
TAF3-r | TGACGGAATTTGGTGTGGGAAGG |
TFF1-f | GCCCCCCGTGAAAGACA |
TFF1-r | CGTCGAAACAGCAGCCCTTA |
TMPRSS2-f | TGTGGTCCCTTCCAATGCTGTG |
TMPRSS2-r | TGCTCATGGTTATGGCACTTGGC |
TNF-f | CCAGGCAGTCAGATCATCTTCTCG |
TNF-r | ATCTCTCAGCTCCACGCCATTG |
TP53-f | AGTGGAAGGAAATTTGCGTGTGG |
TP53-r | TGGTACAGTCAGAGCCAACCTC |
VEGFA-f | AGGGCAGAATCATCACGAAGTGG |
VEGFA-r | AGGGTCTCGATTGGATGGCAGTAG |
ACTINalpha-f | CAGGACCGTGTGGAGCAGATTG |
ACTINalpha-r | CAGATTGTCCCACTGGTCACAG |
B2M-f | TGAGTATGCCTGCCGTGTGAAC |
B2M-r | TGCTGCTTACATGTCTCGATCCC |