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Fig. 1 | Cancer Cell International

Fig. 1

From: DMD transcripts in CRL-2061 rhabdomyosarcoma cells show high levels of intron retention by intron-specific PCR amplification

Fig. 1

Analyses of transcripts in CRL-2061 cells. MK molecular size markers. a PCR amplification of the PAX3/FOXO1 fusion gene transcript. The junction of the fusion gene transcript was PCR amplified. The expected size product was obtained from CRL-2061 cells (CRL), but not from muscle (M). As a control, GAPDH was also amplified (GAPDH). b PCR amplification of DMD cDNA from CRL-2061 cells. DMD cDNA was PCR amplified as 20 separate fragments. Of the 16 products shown, 15 were of the same sizes in CRL-2061 (CRL) cells and muscle (M). Two products were obtained after amplifying exons 18 from both the CRL and M samples. The major band consisted of exons 18, and the minor, but larger band showed insertion of exon 1a between exons 1 and 2(*). The numbers above the electrophoregrams indicate the 5′ and 3′ exons of each amplified fragment

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