Skip to main content
Fig. 5 | Cancer Cell International

Fig. 5

From: DMD transcripts in CRL-2061 rhabdomyosarcoma cells show high levels of intron retention by intron-specific PCR amplification

Fig. 5

Schematic illustration of two methods of restoring dystrophin expression in CRL-2061 cells. A schematic representation of the DMD transcript shows the three last exons and three representative upstream exons (pre-mRNA). Splicing of the pre-mRNA produces mRNA with intron retention (thick bar) and exon 78 skipping (box at the top of the exon structure). This mRNA, with two defects, cannot produce dystrophin. Intron retention may be abolished by antisense oligonucleotide-mediated intron removal. However, this protein product differs at its C-terminal from dystrophin (grey box; mRNA with one defect). Splicing of exon 78 may result in the production of complete dystrophin mRNA. Boxes and lines represent exons and introns, respectively. The number in each box indicates exon number. Exon boxes are not drawn to scale

Back to article page
\