Fig. 3

The expression of endothelial cell miRNA after co-culture with exosomes and miR-146b-5p can inhibit the migration of endothelial cells. a Quantification of individual miRNAs in HUVECs with or without exosomes derived from tumor-associated macrophages. The y-axis represents the relative miRNA expression level. The results are presented as the mean ± SD. miRNA expression in HUVECs with or without exosomes derived from tumor-associated macrophages. When HUVECs were co-cultured with exosomes derived from tumor-associated macrophages, a modest but statistically significant increase of miRNA expression was observed for miR-146b (*P < 0.05), miR-21 (*P < 0.05), miR-24 (*P < 0.05), and miR-132 (*P < 0.05). b, c Migration assay: the number of cells that migrate through the membrane to the lower chamber was measured with calcein-AM (green).Cells in the lower chamber were counted in three random microscopic fields using an inverted microscope. The ability of cells to migrate is significantly suppressed by the addition of exosomes derived from TAMs or by the mimics of hsa-miR-146b-5p