Fig. 5

LncRNA HOXA11-AS epigenetically silenced miR-200b transcription by binding to EZH2 and DNMT1 in NSCLC cells. (a) ChIP assays of EZH2 and DNMT1 occupancy binding in the miR-200b promoter was detected by qRT-PCR assays in A549 and H1299 cells, IgG as a negative control. (b, c) ChIP assays of EZH2 and DNMT1 occupancy binding in the miR-200b promoter was detected by qRT-PCR assays after silencing lncRNA HOXA11-AS in A549 and H1299 cells, IgG as a negative control. (d, e) western blot analysis was used to determine the expression of E-cadherin, N-cadherin and Vimentin by transfecting si-NC, si-HOXA11-AS or si-HOXA11-AS+ miR-200b inhibitor into A549 and H1299 cells. **P < 0.05, Mean ± SD was shown. Data show a representative of three independent experiments