Fig. 5

TSA reduced the HDAC1 and HDAC2 expression. MCF-7 cells were treated with 10–10,000 ng/ml TSA for 24 h. Relative mRNA expression level of HDAC1, HDAC2 and HDAC3 was measured using qRT-PCR where β-actin was used as housekeeping gene and normalised to untreated. Data were collected from n = 4 independent experiments, presented as mean ± SEM. Unpaired Student’s t test *p < 0.05 and ***p < 0.001