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Fig. 3 | Cancer Cell International

Fig. 3

From: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells

Fig. 3

Western blot analysis of CRISPR/Cas9 genome edited Hep3B cells. a Representative Western blot of n = 4 independent experiments. Untransfected (UT), CRISPR/Cas9-Target 1 (T1) or CRISPR/Cas9-Target 2 (T2) transfected Hep3B cells were cultured under normoxic (N) conditions or exposed to hypoxia (H) for 8 h. Protein masses are indicated in kDa. b Densitometry of ARNT protein level (corresponding to a) normalised to Lamin A/C. Values are presented as mean ± SEM of n = 4 independent experiments

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