Table 1 Materials
From: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells
Antibodies | Description | Application |
|---|---|---|
Anti-ARNT | Mouse monoclonal, clone 2B10, #NB300-525, Novus Biologicals | ChIP, WB |
Anti-HIF-1α | Mouse monoclonal, clone H1α67, #NB100-123, Novus Biologicals | ChIP |
Anti-HIF-1α | Mouse monoclonal, clone 54, #610959, BD Transduction Laboratories™ | WB |
Anti-histone H3 | Rabbit monoclonal, clone D2B12, #4620, Cell Signaling Technology | ChIP |
Anti-Lamin A/C | Goat polyclonal, #sc-6215, Santa Cruz | WB |
IgG | Isotype control, rabbit, #2729, Cell Signaling Technology | ChIP |
Oligos | Sequence | |
|---|---|---|
Target 1 top | GATTACAGGCATGCGCCACCACGCCGTTTT | |
Target 1 bottom | GGCGTGGTGGCGCATGCCTGTAATCCGGTG | |
Target 2 top | TTCGAACCCCTGGCCACAGGTGATCGTTTT | |
Target 2 bottom | GATCACCTGTGGCCAGGGGTTCGAACGGTG |
Primer | Sequence | |
|---|---|---|
Region 1 for | CAACGTCGTGAAACTCCATC | |
Region 1 rev | TGCCTCAGTCTCCTGAGTAG | |
Region 2 for | ACGGAGTTTCGCTCTTGTTG | |
Region 2 rev | CCTGTAATCCCAGCTTCTTG | |
Region 3 for | TGCCTCAGCCTCCCAAGAAG | |
Region 3 rev | CGCGTCTGTAATCCCAGCAC | |
RPL30 | #7014, Cell Signaling Technology | |
U6 sequencing | GGACTATCATATGCTTACCG | |
VEGF HRE for | CAGTTCCCTGGCAACATCTG | |
VEGF HRE rev | CAGTGACTGGGAGGGAAGAG |