Fig. 5

Effect of specific inhibitors of Wnt, Hedgehog, PI3k, NF-κB, JNK, ERK, MAPK, and Notch on Bcl-2 expression that was decreased by the combination treatment of curcumin and MMC. a MCF-7 BCSCs were treated with specific inhibitors of Wnt, Hedgehog, PI3k, NF-κB, JNK, ERK, MAPK, and Notch for 12 h. The expression of Wnt, Hedgehog, PI3k, NF-κB, JNK, ERK, MAPK, and Notch was observed through western blot analysis. MCF-7BCSCs were plated and preincubated for 2 h in the presence of b PFK115-584 and cyclopamine; c LY294002 and PDTC. Cells were then treated with 5 μmol/L curcumin and 0.1 μmol/L MMC alone or together for 7 days. Western blot analysis was then performed. The density ratio of proteins to GAPDH is shown as relative expression. Values are expressed as the mean ± SD (n = 3). Experiments were repeated with similar results. *P < 0.05 vs. specific inhibitors alone