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Fig. 7 | Cancer Cell International

Fig. 7

From: Curcumin reduces mitomycin C resistance in breast cancer stem cells by regulating Bcl-2 family-mediated apoptosis

Fig. 7

Effect of specific inhibitors of Wnt (PFK115-584) and PI3k (LY294002) on cell survival and apoptosis that was decreased and induced by the combination treatment of curcumin and MMC. MCF-7 BCSCs were pretreated for 2 h with or without 10 μmol/L PFK115-584 and LY294002 before cells were treated with 5 μmol/L curcumin and 0.1 μmol/L MMC alone or together. The associated proteins of the a Wnt and b PI3k signaling pathway were detected using western blot analysis. Cells were treated for 3, 5, and 7 days. MTT assay (c) and flow cytometry analysis (D) were performed as described in the methods section. Values are presented as the mean ± SD from three independent experiments. *P < 0.05 vs. the combination treatment of curcumin and MMC

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