Fig. 4

Dual EGFR and BRAF blockade prevents EGFR feedback activation and induces prolonged suppression of ERK signaling in BRAF V600E thyroid carcinoma cells. a AKT, phosphoAKT, EGFR, phosphoEGFR, ERK and phosphoERK immunoblot analysis in BRAF V600E FRO, BCPAP, and BHT101 thyroid carcinoma cell lines exposed to 10 μM PLX4032 or 10 μM gefitinib or the combination of both agents for 4 (BCPAP cells), 6 (FRO cells) or 8 (BHT101 cells) h. b ERK and phosphoERK immunoblot analysis in BRAF V600E FRO, BCPAP and BHT101 thyroid carcinoma cell lines exposed to 10 μM PLX4032 or 10 μM gefitinib or the combination of both agents for 15 h (24 h in BHT101 cells)