Fig. 2

Validation of NUDT1 as a direct target of miR-485-5p in GC cells. a The predicted binding sites and base-pairing interaction between miR-485-5p and the 3′UTR of NUDT1 mRNA. b, c Direct recognition of NUDT1 by miR-485-5p. HEK-293T cells were co-transfected with firefly luciferase reporters containing either WT or mutant NUDT1 3′UTR with miR-485-5p mimics or inhibitors. d, e Quantitative RT-PCR analysis of miR-485-5p levels in GC cells transfected with miR-485-5p mimics (d) or inhibitors (e). f The regulation of NUDT1 expression by miR-485-5p in GC cells. g Quantitative analysis of f. h Quantitative RT-PCR analysis of NUDT1 mRNA levels in GC cells transfected with miR-485-5p mimics or inhibitors. *P < 0.05; **P < 0.01; ***P < 0.001