Table 1 Primers used for PCR amplification
From: Sporadic PCDH18 somatic mutations in EpCAM-positive hepatocellular carcinoma
Gene | Exon number | Primer sequence |
|---|---|---|
PCDH18 | Exon 1 | Forward 5′-TAATCTGGGAAGCAAGGGGAC-3′ |
|  |  | Reverse 5′-ACGACCAAACAAACGCAAGG-3′ |
|  | Exon 2 | Forward 5′-CACTGTCTCCTGCCTCACTG-3′ |
|  |  | Reverse 5′-ATAGTTGGTAGCTGGCTGCG-3′ |
|  | Exon 3 | Forward 5′-GGCTGTATCGGATGAGGTGG-3′ |
|  |  | Reverse 5′-CCAGCAGGTCTCTCAGCTTC-3′ |
|  | Exon 4 | Forward 5′-CAGTGGCTAGTTTCTACACGAC3′ |
|  |  | Reverse 5′-TCACACCTAGTTCTTCCCACG-3′ |