Fig. 3

Mitochondrial depolarization was involved in apoptosis induced by combined crocetin and cisplatin treatment. KYSE-150 cells were treated with crocetin or/and cisplatin for 48 h, stained with Rh123 solution, and observed under a fluorescence microscope. a Control; b 200 μmol/L of crocetin; c 2 μg/L of cisplatin; d 200 μmol/L of crocetin + 2 μg/L of cisplatin. e Mean fluorescence intensity analysis. Each bar represents the mean ± SEM, *p < 0.05 compared with the control group; ^# p < 0.05 compared with the combined crocetin and cisplatin group. Scale bar = 100 μm