Fig. 3

MIAT functioned as miR-132 sponge in colon cancer cells. a The correlation between MIAT-1 and miR-132 expression in 30 CRC tissues. b LOVO cells were transfected with si-control, si-MIAT-1 or si-MIAT-2 for 48 h, miR-132 expression was measured. c Sequence alignment of miR-132 with the putative binding sites within the wild-type regions of MIAT-1. d LOVO cells were co-transfected with miR-132 mimic and MIAT-1-WT vector or MIAT-1-MUT vector for 48 h, the luciferase activity was measured. e WT and the mutated forms of miR-132 sequence were shown. f Detection of MIAT using real-time PCR in the sample pulled down by biotinylated miR-132. g Detection of miR-132 using real-time PCR in the sample pulled down by biotinylated MIAT probe. **P < 0.01, compared to si-control, Pre-NC or Bio-NC