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Fig. 3 | Cancer Cell International

Fig. 3

From: Pharmacological inhibition of Bmi1 by PTC-209 impaired tumor growth in head neck squamous cell carcinoma

Fig. 3

PTC-209 inhibited cell proliferation and induced cell cycle arrest, cell apoptosis and enhanced chemosensitivity in HNSCC cells. a Cell proliferation was remarkably suppressed in Cal27 and FaDu cells upon treatment with PTC-209 (10 μM) as measured by MTT assay. b Cell viability was more significantly impaired in cells treated with PTC-209 (10 μM) pus 5-FU (2.5 μg/ml) or cisplatin (2.5 μg/ml) than those treated with single agent. c Increased percentages of cells in G1 stage was observed in Cal27 treated with PTC-209 (10 μM) for 48 h. d Increased percentages of cell undergoing apoptosis (both early and late apoptosis) were detected in cells treated with PTC-209 (10 μM) as compared to control (vehicle-treated cells) as assayed by Annexin V-PI double staining. e The abundances of apoptosis marker cleave-PARP were concomitantly increased after PTC-209 exposure (10 μM) for 48 h. Representative images are shown. Data showed here are mean ± SD from three independent experiments, *p < 0.05, **p < 0.01, Student’s t test and ANOVA analyses

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