Fig. 1
From: ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a
ROR2 participates in Wnt5a-induced osteosarcoma cell migration. a Stable ROR2 knockdown MG-63 cells and osteosarcoma U2OS cells transfected with ROR2-siRNA or scrambled siRNA were subjected to Western blotting assays. The expression of ROR2 in MG-63 and U2OS cells was significantly knockdown by specific shRNAs or siRNAs targeting ROR2. GAPDH was used as an internal control. b and c Stable ROR2 knockdown MG-63 cells and osteosarcoma U2OS cells transfected with ROR2-siRNA or scrambled siRNA were subjected to wound healing assays. Cells incubated with 100 ng/mL Wnt5a were allowed to migrate for 12 h. Data were presented as mean ± SD of 5 determinations. The relative migration distance was normalized to the average value of scrambled group