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Fig. 2 | Cancer Cell International

Fig. 2

From: ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a

Fig. 2

Knockdown of ROR2 decreases Wnt5a-induced RhoA activity and cell migration. a Stable ROR2 knockdown MG-63 cells and osteosarcoma U2OS cells transfected with ROR2-siRNA or scrambled siRNA were incubated with 100 ng/mL Wnt5a and harvested at 30 min after the start of Wnt5a treatment. The determination of RhoA activity was described in “Methods”. Data were presented as mean ± SD of 5 determinations. The relative RhoA activity was normalized to the average value of scrambled group. b, c, and d Stable ROR2 knockdown MG-63 cells (b, c) and osteosarcoma U2OS cells (b, d) transfected with ROR2-siRNA and/or RhoA-V14 (RhoA-CA), were subjected to wound healing assays. Cells incubated with 100 ng/mL Wnt5a were allowed to migrate for 12 h. Data were presented as mean ± SD of 5 determinations. The relative migration distance was normalized to the average value of the control group

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