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Fig. 3 | Cancer Cell International

Fig. 3

From: ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a

Fig. 3

Overexpression of ROR2 increases Wnt5a-induced RhoA activity and cell migration. a Human osteosarcoma MG-63 and U2OS cells, transfected with ROR2-Flag or vectors (Ctrl), were incubated with 100 ng/mL Wnt5a and harvested at 30 min after the start of Wnt5a treatment. The determination of RhoA activity was described in “Methods”. Data were presented as mean ± SD of 5 determinations. The relative RhoA activity was normalized to the average value of scrambled group. b and c MG-63 and U2OS cells, transfected with ROR2-Flag and/or RhoA-siRNA, were subjected to wound healing assays. Cells incubated with 100 ng/mL Wnt5a were allowed to migrate for 12 h. Data were presented as mean ± SD of 5 determinations. The relative migration distance was normalized to the average value of the control group. Ns no significance

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