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Fig. 4 | Cancer Cell International

Fig. 4

From: ROR2 receptor promotes the migration of osteosarcoma cells in response to Wnt5a

Fig. 4

PI3Kα and Akt acts as the downstream of Wnt5a/ROR2 signaling. a and b Human osteosarcoma MG-63 cells, transfected with ROR2-Flag or ROR2-siRNA, were incubated with 100 ng/mL Wnt5a and harvested at 15 min after the start of Wnt5a treatment. The expression and activity of Akt were determined by Western blotting assays. Data were presented as mean ± SD of 3 determinations. The ratio of p-Akt and total Akt was normalized to the average value of control group. c and d MG-63 cells, transfected with ROR2-Flag or vectors, were treated with 1 nmol/L HS-173 (PI3Kα inhibitor), 10 nmol/L MK-2206 (Akt inhibitor), 10 nmol/L A-674563 (Akt1 inhibitor), or 10 nmol/L CCT128930 (Akt2 inhibitor) for 1 h, then subjected to wound healing assays. Cells incubated with 100 ng/mL Wnt5a were allowed to migrate for 12 h. Data were presented as mean ± SD of 5 determinations. The relative migration distance was normalized to the average value of control group

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