Fig. 2

The levels of RAC3 expression affect the response to chemotherapeutic drugs: a–d Knocking down efficiency of shRAC3 in HCT 116 cell line (a, b) and overexpression of RAC3 in LoVo cells (c, d) were determined by qPCR normalized with GAPDH mRNA (a, c) and Western blot where RDU correspond to the average of densitometry units respect to the Tubulin expression, obtained in three independent experiments (b, d). e–h Cell viability was determined by crystal violet staining and IC₅₀ doses were calculated with GraphPad software. CRC cells were treated with FUra (0–150 μM) for 72 h (e, g) or Oxa (0–50 μM) for 24 h (f, h) p < 0.001 IC₅₀ HCT 116 shRAC3 or LoVo RAC3 respect to HCT 116 or LoVo wt and control for FUra in HCT 116 and LoVo and Oxa in LoVo cells and p < 0.01 IC₅₀ HCT 116 shRAC3 respect to HCT 116 for Oxa