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Fig. 2 | Cancer Cell International

Fig. 2

From: CMTM5 is downregulated and suppresses tumour growth in hepatocellular carcinoma through regulating PI3K-AKT signalling

Fig. 2

Overexpression of CMTM5 inhibits HCC cell growth and metastasis in vitro. a Lentivirus vector pLenti6.3-CMTM5-IRES-EGFP and the mock control lentivirus were transfected into Huh7 cells. Transfection efficiency was confirmed by qRT-PCR and western blotting. b Cell growth was assessed by time course CCK-8 assay. c Cell apoptosis was determined by flow cytometry using the Annexin V/PI staining. d Cell metastatic and invasion was assessed by Transwell assay. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared with Huh7-Mock group (One-Way ANOVA with Dunnett’s test)

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