Fig. 1

Quantification of rhodamine-phalloidin binding and summation of CPCR deleterious amino acids in NNK treated, WM9 subclones. Quantification of phalloidin binding is described in “Materials and methods”. See also Table 1 and Additional file 8. The p value for comparison of deleterious amino acids, for the high and low phalloidin-binding subclones is < 0.0004. However, there is no significant difference between the two types of subclones in terms of total unique and shared CPCR mutations, i.e., mutations shared with another subclone in the same high or low phalloidin-binding group. Thus, the high phalloidin-binding subclones had a total of 357 unique and shared mutations and the low phalloidin-binding subclones had a total of 569 mutations, in the CPCR set. The HUGO symbols for the CPCR set, for both Figs. 1 and 6, were obtained from Ref. [25] are: ANK2, APC, COL11A1, DNAH10, DNAH11, DNAH3, DNAH5, DNAH7, DNAH8, DSCAM, DST, FAT3, FAT4, FBN2, FGFR1, FLG, MUC16, MUC17, MUC4, NEB, NEFH, NF1, PCDH15, PCDHAC2, PCDHGC5, PCLO, PKHD1, PLEC, RELN, SPTA1, SPTAN1, SSPO, SYNE1, SYNE2, TTN, and XIRP2