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Fig. 1 | Cancer Cell International

Fig. 1

From: Increased expression of MARCH8, an E3 ubiquitin ligase, is associated with growth of esophageal tumor

Fig. 1

Increased expression of MARCH8 in ESCC tissues. a Boxplot diagram displaying differential expression of MARCH8 mRNA in cancer versus distant matched non-malignant tissues. b Receiver operating characteristic curve of MARCH8 for discriminating ESCC tissues from distant matched non-malignant tissues. c–k Photomicrograph showing the expression of MARCH8 protein in esophageal tissue sections. c Shows no detectable MARCH8 staining in distant matched non-malignant tissue. d Shows nuclear staining in hyperplastic esophageal tissue. e Dysplastic lesion showing nuclear MARCH8 protein. f Well-differentiated SCC showing nuclear MARCH8 staining. g Moderately-differentiated SCC showing predominantly nuclear and faint cytoplasmic MARCH8 staining. h Poorly-differentiated SCC showing both nuclear and cytoplasmic staining. i Represents the negative control in which the ESCC tissue section was not treated with primary rabbit anti-MARCH8 antibody. j Shows negative control KYSE-410 cells wherein no primary antibody was added. k KYSE-410 cells showing MARCH8 protein expression in the cytoplasm and nucleus of the cells. l ROC analysis for MARCH8 protein expression in ESCC tissues. m Western blot for MARCH8 protein detection in nucleus as well as cytoplasmic fractions. Size bar = 100 µm (c–k)

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