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Fig. 2 | Cancer Cell International

Fig. 2

From: Increased expression of MARCH8, an E3 ubiquitin ligase, is associated with growth of esophageal tumor

Fig. 2

Nuclear and cytoplasmic localization of MARCH8 protein in KYSE-410 cells. Cells were incubated with anti-MARCH8 antibody which was visualized with Alexa Fluor-488 conjugated secondary antibody and counterstained with either DAPI (a, b) or PI (c, d) for nuclear staining. Nuclear localization was recognized in the merge image by either cyan staining produced by the blending of green signal from Alexa Fluor-488 and blue signal from DAPI (a) or yellow staining by merging of green signal from Alexa Fluor-488 and red signal from PI (c). b, d Represent negative control wherein no primary antibody was added. Size bar = 45 µm (a, b) and 100 µm (c, d)

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