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Fig. 3 | Cancer Cell International

Fig. 3

From: Increased expression of MARCH8, an E3 ubiquitin ligase, is associated with growth of esophageal tumor

Fig. 3

a Relative expression of MARCH8 mRNA in MARCH8 (50 nmol/l) silencing group and negative control group (*p < 0.05). b Western blot showing downregulation of MARCH8 protein after 48 h of transfection with 50 nmol/l of MARCH8 siRNA (1: scrambled siRNA treated and 2: MARCH8 siRNA treated); **p = 0.01. c Cell growth Inhibitory assay. The inhibitory rate of MARCH8 siRNA treated cells was higher than scrambled siRNA treated cells (*p ≤ 0.05 and ***p < 0.001). d Colony formation assay. The number of colonies formed by MARCH8 siRNA treated cells were 215 ± 6.36 (mean ± SD) as compared to 412 ± 6.36 (mean ± SD) in scrambled siRNA treated or 444 ± 26.16 (mean ± SD) in untreated cells (**p < 0.01). e Scratch assay to detect migration ability of KYSE410 cells. The wound healing capacity of MARCH8 siRNA treated cells was lower than scrambled siRNA treated and untreated cells (**p < 0.01 and ***p < 0.001). Size bar = 500 µm

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