Fig. 2

Measurement of Gli1 and MGMT expression by real-time quantitative PCR (a) and western blot analysis (b) in the three GBM lines, including A172, U251, and U87. The expression of the ubiquitously expressed β-actin gene was measured as the control. The MGMT promoter methylation status was also as showed (c). The lane labelled as ‘M’ means methylated; ‘U’ stands for unmethylated. Gli1 expression was increased by pcDNA3.1-Gli1 transfection in A172 cells measured at mRNA and protein levels since 48 h and thereafter (d–f), as well as MGMT expression and promoter status. When exposed to TMZ treatment, cell viability in the A172 cells transfected with pcDNA3.1-Gli1 was significantly higher than that in the control since 96 h and thereafter (g). The percentage of apoptotic cells at 120 h after pcDNA3.1-Gli1 transfection was significantly lower than in the control cells (h). *p < 0.05; **p < 0.01